Dr. Humayun completed his medical degree at Duke University Medical School. While in his residency training at Duke Eye Center, Dr. Humayun earned his doctorate in Biomedical Engineering at the University of North Carolina at Chapel Hill. He completed his fellowship training in vitreoretinal disease at the Wilmer Ophthalmological Institute at Johns Hopkins.

At Wilmer Dr. Humayun was Associate Professor and Director of the Intraocular Retinal Prosthesis Lab. The focus of the lab was to develop a retinal implant for the blind. He has had a close working relationship with Biomedical Engineering and a number of national labs such as Naval Research Laboratory and Oakridge National Laboratory.